Primers Available
Organism:Naegleria fowleri mitochondrion
Repeat:(T)12
Start:22909 End:22920
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TACTCGATGAATCTGCACGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATGGCACCTATCATTGCAGC | |||||||||||||
| Tm_F_1 | 59.823 | Size_F_1 | 20 | Tm_R_1 | 61.034 | Size_R_1 | 20 | PRODUCT Size | 121 bp | Primer Start | 22844 | Primer End | 22964 |
| FORWARD_PRIMER_2 | |||||||||||||
| TACTCGATGAATCTGCACGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGAATAGCCTTGCTTTTCGTT | |||||||||||||
| Tm_F_2 | 59.823 | Size_F_2 | 20 | Tm_R_2 | 59.75 | Size_R_2 | 21 | PRODUCT Size | 152 bp | Primer Start | 22844 | Primer End | 22995 |
| FORWARD_PRIMER_3 | |||||||||||||
| TACTCGATGAATCTGCACGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGAATAGCCTTGCTTTTCGT | |||||||||||||
| Tm_F_3 | 59.823 | Size_F_3 | 20 | Tm_R_3 | 58.468 | Size_R_3 | 20 | PRODUCT Size | 152 bp | Primer Start | 22844 | Primer End | 22995 |