Primers Available
Organism:Naegleria fowleri mitochondrion
Repeat:(T)10ggaattctaaaaaatatacaatagatgaaataaaagaacaatggaagaaagataggattattaattatatta(AAG)4
Start:40088 End:40181
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGAAACCTATACATACACTGCTTCTTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCTTCCATTGATGTAAACATATAAAC | |||||||||||||
| Tm_F_1 | 58.863 | Size_F_1 | 27 | Tm_R_1 | 57.255 | Size_R_1 | 26 | PRODUCT Size | 280 bp | Primer Start | 39968 | Primer End | 40247 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGAAACCTATACATACACTGCTTCTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCTTCCATTGATGTAAACATATAAAC | |||||||||||||
| Tm_F_2 | 57.696 | Size_F_2 | 26 | Tm_R_2 | 57.255 | Size_R_2 | 26 | PRODUCT Size | 280 bp | Primer Start | 39968 | Primer End | 40247 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGAAACCTATACATACACTGCTTCTTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTCCATTGATGTAAACATATAAACAAA | |||||||||||||
| Tm_F_3 | 58.863 | Size_F_3 | 27 | Tm_R_3 | 58.062 | Size_R_3 | 27 | PRODUCT Size | 278 bp | Primer Start | 39968 | Primer End | 40245 |