Primers Available
Organism:Naegleria fowleri mitochondrion
Repeat:(T)10actccgcacgtagatttagcttttttaagtgtggagcatataatgagagatgttaattacggatggttggttagatatattcatgcaaacggagcatcg(T)11
Start:19238 End:19357
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGGAATTTTGGTTTTATGTCTGC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCACACTAATGTTCTTGGGAAA | |||||||||||||
| Tm_F_1 | 60.226 | Size_F_1 | 23 | Tm_R_1 | 58.994 | Size_R_1 | 22 | PRODUCT Size | 255 bp | Primer Start | 19172 | Primer End | 19426 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGGAATTTTGGTTTTATGTCTGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ACCACACTAATGTTCTTGGGAAA | |||||||||||||
| Tm_F_2 | 60.226 | Size_F_2 | 23 | Tm_R_2 | 59.791 | Size_R_2 | 23 | PRODUCT Size | 256 bp | Primer Start | 19172 | Primer End | 19427 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGGAATTTTGGTTTTATGTCTGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AAACCACACTAATGTTCTTGGGA | |||||||||||||
| Tm_F_3 | 60.226 | Size_F_3 | 23 | Tm_R_3 | 59.791 | Size_R_3 | 23 | PRODUCT Size | 258 bp | Primer Start | 19172 | Primer End | 19429 |