Primers Available
Organism:Naegleria fowleri mitochondrion
Repeat:(T)10
Start:4750 End:4759
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AACAAAGAAACGAATGTTTTTCAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTGTCCATACTTAGAAACACGAAAA | |||||||||||||
| Tm_F_1 | 59.149 | Size_F_1 | 24 | Tm_R_1 | 59.178 | Size_R_1 | 25 | PRODUCT Size | 259 bp | Primer Start | 4671 | Primer End | 4929 |
| FORWARD_PRIMER_2 | |||||||||||||
| CAAAGAAACGAATGTTTTTCAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTGTCCATACTTAGAAACACGAAAA | |||||||||||||
| Tm_F_2 | 57.069 | Size_F_2 | 22 | Tm_R_2 | 59.178 | Size_R_2 | 25 | PRODUCT Size | 257 bp | Primer Start | 4673 | Primer End | 4929 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGGAGAGTTAAAAACAAAGAAACG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGTCCATACTTAGAAACACGAAAAA | |||||||||||||
| Tm_F_3 | 58.942 | Size_F_3 | 24 | Tm_R_3 | 59.178 | Size_R_3 | 25 | PRODUCT Size | 270 bp | Primer Start | 4659 | Primer End | 4928 |