Primers Available
Organism:Naegleria fowleri mitochondrion
Repeat:(T)10
Start:37417 End:37426
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGATGCAAGAAATCCATTCCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CATTCCAATGCCCCTTTTT | |||||||||||||
| Tm_F_1 | 59.519 | Size_F_1 | 21 | Tm_R_1 | 59.748 | Size_R_1 | 19 | PRODUCT Size | 201 bp | Primer Start | 37341 | Primer End | 37541 |
| FORWARD_PRIMER_2 | |||||||||||||
| GATGCAAGAAATCCATTCCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CATTCCAATGCCCCTTTTT | |||||||||||||
| Tm_F_2 | 58.494 | Size_F_2 | 20 | Tm_R_2 | 59.748 | Size_R_2 | 19 | PRODUCT Size | 200 bp | Primer Start | 37342 | Primer End | 37541 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGATGCAAGAAATCCATTCCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACTATTCCCATTCCAATGCC | |||||||||||||
| Tm_F_3 | 59.519 | Size_F_3 | 21 | Tm_R_3 | 58.717 | Size_R_3 | 20 | PRODUCT Size | 209 bp | Primer Start | 37341 | Primer End | 37549 |