Primers Available
Organism:Naegleria fowleri mitochondrion
Repeat:(T)10
Start:33162 End:33171
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CAAAAGAAGAATTCATGCAAGC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CGTGAGCTTCTGGTAGCCAA | |||||||||||||
| Tm_F_1 | 59.013 | Size_F_1 | 22 | Tm_R_1 | 61.499 | Size_R_1 | 20 | PRODUCT Size | 236 bp | Primer Start | 33133 | Primer End | 33368 |
| FORWARD_PRIMER_2 | |||||||||||||
| CAAAAGAAGAATTCATGCAAGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CTACGTGAGCTTCTGGTAGCC | |||||||||||||
| Tm_F_2 | 59.013 | Size_F_2 | 22 | Tm_R_2 | 59.159 | Size_R_2 | 21 | PRODUCT Size | 239 bp | Primer Start | 33133 | Primer End | 33371 |
| FORWARD_PRIMER_3 | |||||||||||||
| CAAAAGAAGAATTCATGCAAGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TACGTGAGCTTCTGGTAGCC | |||||||||||||
| Tm_F_3 | 59.013 | Size_F_3 | 22 | Tm_R_3 | 58.132 | Size_R_3 | 20 | PRODUCT Size | 238 bp | Primer Start | 33133 | Primer End | 33370 |