Primers Available
Organism:Naegleria fowleri mitochondrion
Repeat:(T)10
Start:23478 End:23487
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGGTATTCACTGAGCGTTGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTCCCACTCCATTCCAGGTA | |||||||||||||
| Tm_F_1 | 59.716 | Size_F_1 | 20 | Tm_R_1 | 60.309 | Size_R_1 | 20 | PRODUCT Size | 206 bp | Primer Start | 23332 | Primer End | 23537 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGGTATTCACTGAGCGTTGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TATGCGTTTCCCACTCCATT | |||||||||||||
| Tm_F_2 | 59.716 | Size_F_2 | 20 | Tm_R_2 | 60.331 | Size_R_2 | 20 | PRODUCT Size | 213 bp | Primer Start | 23332 | Primer End | 23544 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGGTATTCACTGAGCGTTGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AATATGCGTTTCCCACTCCA | |||||||||||||
| Tm_F_3 | 59.716 | Size_F_3 | 20 | Tm_R_3 | 60.331 | Size_R_3 | 20 | PRODUCT Size | 215 bp | Primer Start | 23332 | Primer End | 23546 |