Primers Available
Organism:Naegleria fowleri mitochondrion
Repeat:(T)10
Start:11597 End:11606
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GGTTCGAGTCCTACTGGGTTC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCAAACGATGGTAAATCGCA | |||||||||||||
| Tm_F_1 | 59.987 | Size_F_1 | 21 | Tm_R_1 | 60.073 | Size_R_1 | 20 | PRODUCT Size | 159 bp | Primer Start | 11556 | Primer End | 11714 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTTGGGAAAACGCTGTCTATG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCAAACGATGGTAAATCGCA | |||||||||||||
| Tm_F_2 | 60.117 | Size_F_2 | 21 | Tm_R_2 | 60.073 | Size_R_2 | 20 | PRODUCT Size | 182 bp | Primer Start | 11533 | Primer End | 11714 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTGGGAAAACGCTGTCTATG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCAAACGATGGTAAATCGCA | |||||||||||||
| Tm_F_3 | 58.771 | Size_F_3 | 20 | Tm_R_3 | 60.073 | Size_R_3 | 20 | PRODUCT Size | 181 bp | Primer Start | 11534 | Primer End | 11714 |