Primers Available
Organism:Naegleria fowleri mitochondrion
Repeat:(ATT)4
Start:8886 End:8897
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GAAGTTCGTCTAAAGACGTAATAGGTG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TAGTTGGCGCAACGTTACCA | |||||||||||||
| Tm_F_1 | 60.026 | Size_F_1 | 27 | Tm_R_1 | 62.51 | Size_R_1 | 20 | PRODUCT Size | 178 bp | Primer Start | 8744 | Primer End | 8921 |
| FORWARD_PRIMER_2 | |||||||||||||
| GAAGTTCGTCTAAAGACGTAATAGGTG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CAAGAAAATAATAGTTGGCGCA | |||||||||||||
| Tm_F_2 | 60.026 | Size_F_2 | 27 | Tm_R_2 | 59.301 | Size_R_2 | 22 | PRODUCT Size | 189 bp | Primer Start | 8744 | Primer End | 8932 |
| FORWARD_PRIMER_3 | |||||||||||||
| GAAGTTCGTCTAAAGACGTAATAGGTG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGTTGGCGCAACGTTACCAT | |||||||||||||
| Tm_F_3 | 60.026 | Size_F_3 | 27 | Tm_R_3 | 62.73 | Size_R_3 | 20 | PRODUCT Size | 177 bp | Primer Start | 8744 | Primer End | 8920 |