Primers Available
Organism:Naegleria fowleri mitochondrion
Repeat:(A)21
Start:4979 End:4999
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTTTCGTGTTTCTAAGTATGGACAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCTCGAGATGGAATCGAACC | |||||||||||||
| Tm_F_1 | 59.178 | Size_F_1 | 25 | Tm_R_1 | 60.158 | Size_R_1 | 20 | PRODUCT Size | 256 bp | Primer Start | 4905 | Primer End | 5160 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTTTTCGTGTTTCTAAGTATGGACA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCTCGAGATGGAATCGAACC | |||||||||||||
| Tm_F_2 | 59.178 | Size_F_2 | 25 | Tm_R_2 | 60.158 | Size_R_2 | 20 | PRODUCT Size | 257 bp | Primer Start | 4904 | Primer End | 5160 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTTTCGTGTTTCTAAGTATGGACAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCGCTCAAGGATTTTCAGTC | |||||||||||||
| Tm_F_3 | 59.178 | Size_F_3 | 25 | Tm_R_3 | 59.813 | Size_R_3 | 20 | PRODUCT Size | 235 bp | Primer Start | 4905 | Primer End | 5139 |