Primers Available
Organism:Naegleria fowleri mitochondrion
Repeat:(A)15
Start:26996 End:27010
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CGTGGAATAAACAAACGATGC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGCGTCAACTTAATGCTGAGA | |||||||||||||
| Tm_F_1 | 60.37 | Size_F_1 | 21 | Tm_R_1 | 60.401 | Size_R_1 | 21 | PRODUCT Size | 227 bp | Primer Start | 26855 | Primer End | 27081 |
| FORWARD_PRIMER_2 | |||||||||||||
| CGTGGAATAAACAAACGATGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCGTCAACTTAATGCTGAGAAA | |||||||||||||
| Tm_F_2 | 60.37 | Size_F_2 | 21 | Tm_R_2 | 59.541 | Size_R_2 | 22 | PRODUCT Size | 226 bp | Primer Start | 26855 | Primer End | 27080 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCGTGGAATAAACAAACGATG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGCGTCAACTTAATGCTGAGA | |||||||||||||
| Tm_F_3 | 58.544 | Size_F_3 | 21 | Tm_R_3 | 60.401 | Size_R_3 | 21 | PRODUCT Size | 228 bp | Primer Start | 26854 | Primer End | 27081 |