Primers Available
Organism:Naegleria fowleri mitochondrion
Repeat:(A)10
Start:28176 End:28185
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GATGATTTCGCGTTTCACAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CATCCGTACTCCTGTTGGCT | |||||||||||||
| Tm_F_1 | 59.67 | Size_F_1 | 20 | Tm_R_1 | 60.134 | Size_R_1 | 20 | PRODUCT Size | 247 bp | Primer Start | 28004 | Primer End | 28250 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGATTTCGCGTTTCACAAGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CATCCGTACTCCTGTTGGCT | |||||||||||||
| Tm_F_2 | 60.379 | Size_F_2 | 20 | Tm_R_2 | 60.134 | Size_R_2 | 20 | PRODUCT Size | 245 bp | Primer Start | 28006 | Primer End | 28250 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTCGCGTTTCACAAGACGTA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CATCCGTACTCCTGTTGGCT | |||||||||||||
| Tm_F_3 | 60.436 | Size_F_3 | 20 | Tm_R_3 | 60.134 | Size_R_3 | 20 | PRODUCT Size | 241 bp | Primer Start | 28010 | Primer End | 28250 |