Primers Available
Organism:Mesocentrotus nudus mitochondrion
Repeat:(G)19
Start:1189 End:1207
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCCCATCACAACTCCTACCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACATCTGCAACTCCCAAAGC | |||||||||||||
| Tm_F_1 | 59.779 | Size_F_1 | 20 | Tm_R_1 | 60.263 | Size_R_1 | 20 | PRODUCT Size | 164 bp | Primer Start | 1120 | Primer End | 1283 |
| FORWARD_PRIMER_2 | |||||||||||||
| GTCGTTCGGCTTTTCTGGTA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ACATCTGCAACTCCCAAAGC | |||||||||||||
| Tm_F_2 | 60.249 | Size_F_2 | 20 | Tm_R_2 | 60.263 | Size_R_2 | 20 | PRODUCT Size | 133 bp | Primer Start | 1151 | Primer End | 1283 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCGTTCGGCTTTTCTGGTAT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACATCTGCAACTCCCAAAGC | |||||||||||||
| Tm_F_3 | 59.708 | Size_F_3 | 20 | Tm_R_3 | 60.263 | Size_R_3 | 20 | PRODUCT Size | 132 bp | Primer Start | 1152 | Primer End | 1283 |