Primers Available
Organism:Spalacopus cyanus voucher DG657 mitochondrion
Repeat:(ATT)4
Start:2178 End:2189
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCTCGTGAAGAGGCGAGAAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TAGGATTGCGCTGTTATCCC | |||||||||||||
| Tm_F_1 | 60.096 | Size_F_1 | 20 | Tm_R_1 | 60.06 | Size_R_1 | 20 | PRODUCT Size | 269 bp | Primer Start | 2110 | Primer End | 2378 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCTCGTGAAGAGGCGAGAAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGATTGCGCTGTTATCCCTA | |||||||||||||
| Tm_F_2 | 60.096 | Size_F_2 | 20 | Tm_R_2 | 60.06 | Size_R_2 | 20 | PRODUCT Size | 267 bp | Primer Start | 2110 | Primer End | 2376 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCTCGTGAAGAGGCGAGAAT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGTCACCCCAACCAAAATTA | |||||||||||||
| Tm_F_3 | 60.096 | Size_F_3 | 20 | Tm_R_3 | 59.523 | Size_R_3 | 20 | PRODUCT Size | 140 bp | Primer Start | 2110 | Primer End | 2249 |