Primers Available
Organism:Hynobius nebulosus voucher CIB-XM3140 mitochondrion
Repeat:(TAA)4(ATTT)3
Start:4529 End:4552
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGAGGCGGATTAAACCAAAC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGAGTCCGCCTAAGGATATG | |||||||||||||
| Tm_F_1 | 59.938 | Size_F_1 | 20 | Tm_R_1 | 58.622 | Size_R_1 | 20 | PRODUCT Size | 265 bp | Primer Start | 4428 | Primer End | 4692 |
| FORWARD_PRIMER_2 | |||||||||||||
| GAGGCGGATTAAACCAAACA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGAGTCCGCCTAAGGATATG | |||||||||||||
| Tm_F_2 | 59.938 | Size_F_2 | 20 | Tm_R_2 | 58.622 | Size_R_2 | 20 | PRODUCT Size | 264 bp | Primer Start | 4429 | Primer End | 4692 |
| FORWARD_PRIMER_3 | |||||||||||||
| GATGAGGCGGATTAAACCAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGAGTCCGCCTAAGGATATG | |||||||||||||
| Tm_F_3 | 59.901 | Size_F_3 | 20 | Tm_R_3 | 58.622 | Size_R_3 | 20 | PRODUCT Size | 267 bp | Primer Start | 4426 | Primer End | 4692 |