Primers Available
Organism:Pseudohynobius flavomaculatus voucher CIB-XM2084 mitochondrion
Repeat:(TTA)4
Start:15271 End:15282
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATCGAAATTGGACAAATCGC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CGGTTTACAAGACCGATGCT | |||||||||||||
| Tm_F_1 | 59.907 | Size_F_1 | 20 | Tm_R_1 | 60.132 | Size_R_1 | 20 | PRODUCT Size | 136 bp | Primer Start | 15227 | Primer End | 15362 |
| FORWARD_PRIMER_2 | |||||||||||||
| CATCAAAACAACGCAGCCTA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CGGTTTACAAGACCGATGCT | |||||||||||||
| Tm_F_2 | 59.872 | Size_F_2 | 20 | Tm_R_2 | 60.132 | Size_R_2 | 20 | PRODUCT Size | 252 bp | Primer Start | 15111 | Primer End | 15362 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCGCATCAGCCCTTTATTTC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CGGTTTACAAGACCGATGCT | |||||||||||||
| Tm_F_3 | 60.175 | Size_F_3 | 20 | Tm_R_3 | 60.132 | Size_R_3 | 20 | PRODUCT Size | 120 bp | Primer Start | 15243 | Primer End | 15362 |