Primers Available
Organism:Pseudohynobius flavomaculatus voucher CIB-XM2084 mitochondrion
Repeat:(TAA)4
Start:9509 End:9520
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGAAGCTGCAGCATGATATTG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGTCAAATCCGCACTCGTAT | |||||||||||||
| Tm_F_1 | 59.991 | Size_F_1 | 21 | Tm_R_1 | 59.962 | Size_R_1 | 20 | PRODUCT Size | 278 bp | Primer Start | 9344 | Primer End | 9621 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGAAGCTGCAGCATGATATTG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AAATCCGCACTCGTATGGAG | |||||||||||||
| Tm_F_2 | 59.991 | Size_F_2 | 21 | Tm_R_2 | 60.096 | Size_R_2 | 20 | PRODUCT Size | 274 bp | Primer Start | 9344 | Primer End | 9617 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGCTGCAGCATGATATTGACA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGTCAAATCCGCACTCGTAT | |||||||||||||
| Tm_F_3 | 59.464 | Size_F_3 | 21 | Tm_R_3 | 59.962 | Size_R_3 | 20 | PRODUCT Size | 275 bp | Primer Start | 9347 | Primer End | 9621 |