Primers Available
Organism:Pseudohynobius flavomaculatus voucher CIB-XM2084 mitochondrion
Repeat:(T)11
Start:15923 End:15933
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CACTTGGCCAGCATAACTGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATTAAACAAGCTCCGGGGG | |||||||||||||
| Tm_F_1 | 59.864 | Size_F_1 | 20 | Tm_R_1 | 61.177 | Size_R_1 | 19 | PRODUCT Size | 236 bp | Primer Start | 15884 | Primer End | 16119 |
| FORWARD_PRIMER_2 | |||||||||||||
| TAACTGAGTCGGCCCTCATC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ATTAAACAAGCTCCGGGGG | |||||||||||||
| Tm_F_2 | 60.218 | Size_F_2 | 20 | Tm_R_2 | 61.177 | Size_R_2 | 19 | PRODUCT Size | 223 bp | Primer Start | 15897 | Primer End | 16119 |
| FORWARD_PRIMER_3 | |||||||||||||
| CACTTGGCCAGCATAACTGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AATTAAACAAGCTCCGGGGG | |||||||||||||
| Tm_F_3 | 59.864 | Size_F_3 | 20 | Tm_R_3 | 62.337 | Size_R_3 | 20 | PRODUCT Size | 237 bp | Primer Start | 15884 | Primer End | 16120 |