Primers Available
Organism:Pseudohynobius puxiongensis mitochondrion
Repeat:(TAA)4
Start:4530 End:4541
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCGCGCATATAGGATGAATG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTGGAGGAAGGCCTCCTATT | |||||||||||||
| Tm_F_1 | 59.644 | Size_F_1 | 20 | Tm_R_1 | 60.032 | Size_R_1 | 20 | PRODUCT Size | 219 bp | Primer Start | 4481 | Primer End | 4699 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATCAAACACAGCTGCGAAAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTGGAGGAAGGCCTCCTATT | |||||||||||||
| Tm_F_2 | 59.469 | Size_F_2 | 20 | Tm_R_2 | 60.032 | Size_R_2 | 20 | PRODUCT Size | 258 bp | Primer Start | 4442 | Primer End | 4699 |
| FORWARD_PRIMER_3 | |||||||||||||
| AATCAAACACAGCTGCGAAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTGGAGGAAGGCCTCCTATT | |||||||||||||
| Tm_F_3 | 59.469 | Size_F_3 | 20 | Tm_R_3 | 60.032 | Size_R_3 | 20 | PRODUCT Size | 259 bp | Primer Start | 4441 | Primer End | 4699 |