Primers Available
Organism:Pseudohynobius puxiongensis mitochondrion
Repeat:(T)11
Start:15935 End:15945
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CACTTGGCCAGCATAACTGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATAAACAAGCTCCGGGGG | |||||||||||||
| Tm_F_1 | 59.864 | Size_F_1 | 20 | Tm_R_1 | 59.887 | Size_R_1 | 18 | PRODUCT Size | 235 bp | Primer Start | 15895 | Primer End | 16129 |
| FORWARD_PRIMER_2 | |||||||||||||
| CACTTGGCCAGCATAACTGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AATAAACAAGCTCCGGGGG | |||||||||||||
| Tm_F_2 | 59.864 | Size_F_2 | 20 | Tm_R_2 | 61.177 | Size_R_2 | 19 | PRODUCT Size | 236 bp | Primer Start | 15895 | Primer End | 16130 |
| FORWARD_PRIMER_3 | |||||||||||||
| TAACTGAGTCGGCCCTCATT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATAAACAAGCTCCGGGGG | |||||||||||||
| Tm_F_3 | 59.694 | Size_F_3 | 20 | Tm_R_3 | 59.887 | Size_R_3 | 18 | PRODUCT Size | 222 bp | Primer Start | 15908 | Primer End | 16129 |