Primers Available
Organism:Pseudohynobius puxiongensis mitochondrion
Repeat:(ATA)4
Start:9513 End:9524
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCGCAGCATGATACTGACAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGGTCAAATCCGCACTCATA | |||||||||||||
| Tm_F_1 | 59.704 | Size_F_1 | 20 | Tm_R_1 | 60.864 | Size_R_1 | 20 | PRODUCT Size | 274 bp | Primer Start | 9357 | Primer End | 9630 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCGCAGCATGATACTGACAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CTAAGGGGTCAAATCCGCAC | |||||||||||||
| Tm_F_2 | 59.704 | Size_F_2 | 20 | Tm_R_2 | 61.746 | Size_R_2 | 20 | PRODUCT Size | 279 bp | Primer Start | 9357 | Primer End | 9635 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCGCAGCATGATACTGACAT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TAAGGGGTCAAATCCGCACT | |||||||||||||
| Tm_F_3 | 59.704 | Size_F_3 | 20 | Tm_R_3 | 61.746 | Size_R_3 | 20 | PRODUCT Size | 278 bp | Primer Start | 9357 | Primer End | 9634 |