Primers Available
Organism:Pseudohynobius puxiongensis mitochondrion
Repeat:(A)11
Start:15504 End:15514
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCCACCTGAACCTCTCTCAG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GAACACTTCCGTGGGGTAAA | |||||||||||||
| Tm_F_1 | 59.828 | Size_F_1 | 20 | Tm_R_1 | 59.83 | Size_R_1 | 20 | PRODUCT Size | 246 bp | Primer Start | 15439 | Primer End | 15684 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCCACTTTCTCCAGGCTCTC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GAACACTTCCGTGGGGTAAA | |||||||||||||
| Tm_F_2 | 59.53 | Size_F_2 | 20 | Tm_R_2 | 59.83 | Size_R_2 | 20 | PRODUCT Size | 265 bp | Primer Start | 15420 | Primer End | 15684 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCCACCTGAACCTCTCTCAG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGACTAAAAATCAGCGGGGC | |||||||||||||
| Tm_F_3 | 59.828 | Size_F_3 | 20 | Tm_R_3 | 60.582 | Size_R_3 | 20 | PRODUCT Size | 270 bp | Primer Start | 15439 | Primer End | 15708 |