Primers Available
Organism:Sarcocheilichthys nigripinnis mitochondrion
Repeat:(T)10
Start:16228 End:16237
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CATGCATGATAGAACCAGGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTGTGCCTGCACTCTGAAAT | |||||||||||||
| Tm_F_1 | 58.95 | Size_F_1 | 20 | Tm_R_1 | 59.445 | Size_R_1 | 20 | PRODUCT Size | 236 bp | Primer Start | 16038 | Primer End | 16273 |
| FORWARD_PRIMER_2 | |||||||||||||
| CATGCATGATAGAACCAGGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ATTTGTGCCTGCACTCTGAA | |||||||||||||
| Tm_F_2 | 58.95 | Size_F_2 | 20 | Tm_R_2 | 59.445 | Size_R_2 | 20 | PRODUCT Size | 238 bp | Primer Start | 16038 | Primer End | 16275 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACATACTCCGCATTACCCCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TATTTGTGCCTGCACTCTGAA | |||||||||||||
| Tm_F_3 | 60.214 | Size_F_3 | 20 | Tm_R_3 | 59.478 | Size_R_3 | 21 | PRODUCT Size | 100 bp | Primer Start | 16177 | Primer End | 16276 |