Primers Available
Organism:Sarcocheilichthys nigripinnis mitochondrion
Repeat:(ACAA)3
Start:13503 End:13514
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCCATCATAACAATACCCGC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CTTTTGGTCCCGAGATTTCA | |||||||||||||
| Tm_F_1 | 60.038 | Size_F_1 | 20 | Tm_R_1 | 60.044 | Size_R_1 | 20 | PRODUCT Size | 262 bp | Primer Start | 13412 | Primer End | 13673 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCCATCATAACAATACCCGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AGTCCTTTTGGTCCCGAGAT | |||||||||||||
| Tm_F_2 | 60.038 | Size_F_2 | 20 | Tm_R_2 | 59.935 | Size_R_2 | 20 | PRODUCT Size | 266 bp | Primer Start | 13412 | Primer End | 13677 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCCATCATAACAATACCCGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CGATTGATTGTCCAAGGGTT | |||||||||||||
| Tm_F_3 | 60.038 | Size_F_3 | 20 | Tm_R_3 | 59.79 | Size_R_3 | 20 | PRODUCT Size | 217 bp | Primer Start | 13412 | Primer End | 13628 |