Primers Available
Organism:Pucrasia macrolopha mitochondrion
Repeat:(T)11
Start:914 End:924
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GTTTGCGTGTATGGGGAATC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGGGTTTTTATGCGGTAGGT | |||||||||||||
| Tm_F_1 | 60.199 | Size_F_1 | 20 | Tm_R_1 | 60.075 | Size_R_1 | 20 | PRODUCT Size | 263 bp | Primer Start | 721 | Primer End | 983 |
| FORWARD_PRIMER_2 | |||||||||||||
| GCGTGTATGGGGAATCATCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGGGTTTTTATGCGGTAGGT | |||||||||||||
| Tm_F_2 | 59.78 | Size_F_2 | 20 | Tm_R_2 | 60.075 | Size_R_2 | 20 | PRODUCT Size | 259 bp | Primer Start | 725 | Primer End | 983 |
| FORWARD_PRIMER_3 | |||||||||||||
| CACTGATGCACTTTGGATCG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGGGTTTTTATGCGGTAGGT | |||||||||||||
| Tm_F_3 | 60.263 | Size_F_3 | 20 | Tm_R_3 | 60.075 | Size_R_3 | 20 | PRODUCT Size | 236 bp | Primer Start | 748 | Primer End | 983 |