Primers Available
Organism:Squaliobarbus curriculus x Ctenopharyngodon idella mitochondrion
Repeat:(TA)6
Start:16497 End:16508
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTCGAGAACGTGCAAGCTAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TATTTGGGGAAATTATGCGG | |||||||||||||
| Tm_F_1 | 59.752 | Size_F_1 | 20 | Tm_R_1 | 59.631 | Size_R_1 | 20 | PRODUCT Size | 103 bp | Primer Start | 16446 | Primer End | 16548 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTATCCTTGTCAAACCCCGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TATTTGGGGAAATTATGCGG | |||||||||||||
| Tm_F_2 | 60.301 | Size_F_2 | 20 | Tm_R_2 | 59.631 | Size_R_2 | 20 | PRODUCT Size | 135 bp | Primer Start | 16414 | Primer End | 16548 |
| FORWARD_PRIMER_3 | |||||||||||||
| TATCCTTGTCAAACCCCGAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TATTTGGGGAAATTATGCGG | |||||||||||||
| Tm_F_3 | 60.301 | Size_F_3 | 20 | Tm_R_3 | 59.631 | Size_R_3 | 20 | PRODUCT Size | 134 bp | Primer Start | 16415 | Primer End | 16548 |