Primers Available
Organism:Nuclearia simplex mitochondrion
Repeat:(ATTAA)3
Start:42060 End:42074
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AACGTTTTCAGGGCTTAAATTAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AATTTTCCTCGCCTTTCCAT | |||||||||||||
| Tm_F_1 | 57.512 | Size_F_1 | 23 | Tm_R_1 | 59.909 | Size_R_1 | 20 | PRODUCT Size | 160 bp | Primer Start | 42020 | Primer End | 42179 |
| FORWARD_PRIMER_2 | |||||||||||||
| AACGTTTTCAGGGCTTAAATTAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TAATTTTCCTCGCCTTTCCA | |||||||||||||
| Tm_F_2 | 57.512 | Size_F_2 | 23 | Tm_R_2 | 59.655 | Size_R_2 | 20 | PRODUCT Size | 161 bp | Primer Start | 42020 | Primer End | 42180 |
| FORWARD_PRIMER_3 | |||||||||||||
| AACGTTTTCAGGGCTTAAATTAT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGCGGCGTAATAATTTTCCT | |||||||||||||
| Tm_F_3 | 57.512 | Size_F_3 | 23 | Tm_R_3 | 59.586 | Size_R_3 | 20 | PRODUCT Size | 171 bp | Primer Start | 42020 | Primer End | 42190 |