Primers Available
Organism:Microbotryum cf. violaceum BFL-2013 mitochondrion
Repeat:(AATT)3tacctatttctc(T)10
Start:85475 End:85508
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTCGATTCTGGGAGTTGACC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CAGAAAAACAAGCGCAGAAA | |||||||||||||
| Tm_F_1 | 60.05 | Size_F_1 | 20 | Tm_R_1 | 59.226 | Size_R_1 | 20 | PRODUCT Size | 159 bp | Primer Start | 85375 | Primer End | 85533 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTCGATTCTGGGAGTTGACC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCGCAAAAATAAAAACAGCA | |||||||||||||
| Tm_F_2 | 60.05 | Size_F_2 | 20 | Tm_R_2 | 58.998 | Size_R_2 | 20 | PRODUCT Size | 247 bp | Primer Start | 85375 | Primer End | 85621 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTCGATTCTGGGAGTTGACC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGCGCAAAAATAAAAACAGC | |||||||||||||
| Tm_F_3 | 60.05 | Size_F_3 | 20 | Tm_R_3 | 58.998 | Size_R_3 | 20 | PRODUCT Size | 248 bp | Primer Start | 85375 | Primer End | 85622 |