Primers Available
Organism:Microbotryum cf. violaceum BFL-2013 mitochondrion
Repeat:(AAAAC)3aaaagcagt(A)11
Start:89445 End:89479
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CGCAAAAAGGACAAAACACA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCTGTTGATCGGAAGGGTTC | |||||||||||||
| Tm_F_1 | 59.746 | Size_F_1 | 20 | Tm_R_1 | 60.05 | Size_R_1 | 20 | PRODUCT Size | 271 bp | Primer Start | 89269 | Primer End | 89539 |
| FORWARD_PRIMER_2 | |||||||||||||
| AAACCGCAAAAAGGACAAAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCTGTTGATCGGAAGGGTTC | |||||||||||||
| Tm_F_2 | 59.603 | Size_F_2 | 20 | Tm_R_2 | 60.05 | Size_R_2 | 20 | PRODUCT Size | 275 bp | Primer Start | 89265 | Primer End | 89539 |
| FORWARD_PRIMER_3 | |||||||||||||
| AAAACCGCAAAAAGGACAAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCTGTTGATCGGAAGGGTTC | |||||||||||||
| Tm_F_3 | 59.603 | Size_F_3 | 20 | Tm_R_3 | 60.05 | Size_R_3 | 20 | PRODUCT Size | 276 bp | Primer Start | 89264 | Primer End | 89539 |