Primers Available
Organism:Microbotryum lychnidis-dioicae strain MvSl135HT1 mitochondrion
Repeat:(GGGAA)4
Start:30964 End:30983
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCAATAGCACAAAAATACACAAGC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTCCAATAGATTCGCAGGTTT | |||||||||||||
| Tm_F_1 | 59.962 | Size_F_1 | 24 | Tm_R_1 | 58.707 | Size_R_1 | 21 | PRODUCT Size | 280 bp | Primer Start | 30918 | Primer End | 31197 |
| FORWARD_PRIMER_2 | |||||||||||||
| CACAAGCAATAAATTATCCCTGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CATTCCAATAGATTCGCAGGT | |||||||||||||
| Tm_F_2 | 58.212 | Size_F_2 | 23 | Tm_R_2 | 59.057 | Size_R_2 | 21 | PRODUCT Size | 265 bp | Primer Start | 30935 | Primer End | 31199 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCAATAGCACAAAAATACACAAGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCCAATAGATTCGCAGGTTT | |||||||||||||
| Tm_F_3 | 59.962 | Size_F_3 | 24 | Tm_R_3 | 57.306 | Size_R_3 | 20 | PRODUCT Size | 279 bp | Primer Start | 30918 | Primer End | 31196 |