Primers Available
Organism:Microbotryum lychnidis-dioicae strain MvSl135HT1 mitochondrion
Repeat:(CATTC)3
Start:75366 End:75380
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTGATGGATGACACCGAATTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GATTTGCGTCATCAGTCACA | |||||||||||||
| Tm_F_1 | 59.805 | Size_F_1 | 21 | Tm_R_1 | 58.186 | Size_R_1 | 20 | PRODUCT Size | 275 bp | Primer Start | 75260 | Primer End | 75534 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCTGATGGATGACACCGAAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CGTCATCAGTCACATATGGTCC | |||||||||||||
| Tm_F_2 | 60.747 | Size_F_2 | 20 | Tm_R_2 | 60.263 | Size_R_2 | 22 | PRODUCT Size | 270 bp | Primer Start | 75259 | Primer End | 75528 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCAGCTTAGCCTGATGGATG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CGTCATCAGTCACATATGGTCC | |||||||||||||
| Tm_F_3 | 58.976 | Size_F_3 | 20 | Tm_R_3 | 60.263 | Size_R_3 | 22 | PRODUCT Size | 279 bp | Primer Start | 75250 | Primer End | 75528 |