Primers Available
Organism:Acentrogobius chlorostigmatoides mitochondrion
Repeat:(AAT)4
Start:938 End:949
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AACGAGCATCTGAAGGAGGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CACGGGTATCTTCTCGGTGT | |||||||||||||
| Tm_F_1 | 59.95 | Size_F_1 | 20 | Tm_R_1 | 59.989 | Size_R_1 | 20 | PRODUCT Size | 246 bp | Primer Start | 821 | Primer End | 1066 |
| FORWARD_PRIMER_2 | |||||||||||||
| AGAGGGGAAGAAATGGGCTA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GTGCACCTTCCGGTACACTT | |||||||||||||
| Tm_F_2 | 60.032 | Size_F_2 | 20 | Tm_R_2 | 60.035 | Size_R_2 | 20 | PRODUCT Size | 238 bp | Primer Start | 765 | Primer End | 1002 |
| FORWARD_PRIMER_3 | |||||||||||||
| AACGAGCATCTGAAGGAGGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GACTCGATTTGCACGGGTAT | |||||||||||||
| Tm_F_3 | 59.95 | Size_F_3 | 20 | Tm_R_3 | 59.962 | Size_R_3 | 20 | PRODUCT Size | 257 bp | Primer Start | 821 | Primer End | 1077 |