Primers Available
Organism:Liobagrus kingi mitochondrion
Repeat:(CCT)4
Start:14436 End:14447
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TATGGTAACCCGAAAAACGC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATCCGTAATTCACGTCTCGG | |||||||||||||
| Tm_F_1 | 59.832 | Size_F_1 | 20 | Tm_R_1 | 59.955 | Size_R_1 | 20 | PRODUCT Size | 227 bp | Primer Start | 14333 | Primer End | 14559 |
| FORWARD_PRIMER_2 | |||||||||||||
| GAAAAACGCACCCCCTATTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ATCCGTAATTCACGTCTCGG | |||||||||||||
| Tm_F_2 | 60.184 | Size_F_2 | 20 | Tm_R_2 | 59.955 | Size_R_2 | 20 | PRODUCT Size | 216 bp | Primer Start | 14344 | Primer End | 14559 |
| FORWARD_PRIMER_3 | |||||||||||||
| TAACGACGCACTCATTGACC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATCCGTAATTCACGTCTCGG | |||||||||||||
| Tm_F_3 | 59.722 | Size_F_3 | 20 | Tm_R_3 | 59.955 | Size_R_3 | 20 | PRODUCT Size | 188 bp | Primer Start | 14372 | Primer End | 14559 |