Primers Available
Organism:Filchnerella helanshanensis voucher Z0731 mitochondrion
Repeat:(TTA)4
Start:2827 End:2838
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTTCCCACAACACTTCCTGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGGGTTATTTTGGAGCCATT | |||||||||||||
| Tm_F_1 | 60.536 | Size_F_1 | 20 | Tm_R_1 | 60.02 | Size_R_1 | 20 | PRODUCT Size | 223 bp | Primer Start | 2698 | Primer End | 2920 |
| FORWARD_PRIMER_2 | |||||||||||||
| CACAACACTTCCTGGGGCTA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGGGTTATTTTGGAGCCATT | |||||||||||||
| Tm_F_2 | 61.084 | Size_F_2 | 20 | Tm_R_2 | 60.02 | Size_R_2 | 20 | PRODUCT Size | 218 bp | Primer Start | 2703 | Primer End | 2920 |
| FORWARD_PRIMER_3 | |||||||||||||
| GCTAGCTGGGATACCTCGAC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGGGTTATTTTGGAGCCATT | |||||||||||||
| Tm_F_3 | 58.914 | Size_F_3 | 20 | Tm_R_3 | 60.02 | Size_R_3 | 20 | PRODUCT Size | 202 bp | Primer Start | 2719 | Primer End | 2920 |