Primers Available
Organism:Sinibotia superciliaris haplotype SS1 mitochondrion
Repeat:(AAC)4
Start:10428 End:10439
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACATGGGACAGATCGCCTAC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTGAGAGAGGGTCTGTGGCT | |||||||||||||
| Tm_F_1 | 59.957 | Size_F_1 | 20 | Tm_R_1 | 59.986 | Size_R_1 | 20 | PRODUCT Size | 229 bp | Primer Start | 10317 | Primer End | 10545 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGGACAGATCGCCTACAAAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTGAGAGAGGGTCTGTGGCT | |||||||||||||
| Tm_F_2 | 60.074 | Size_F_2 | 20 | Tm_R_2 | 59.986 | Size_R_2 | 20 | PRODUCT Size | 225 bp | Primer Start | 10321 | Primer End | 10545 |
| FORWARD_PRIMER_3 | |||||||||||||
| CAGGCCTAGCCCTCCTAGTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTGAGAGAGGGTCTGTGGCT | |||||||||||||
| Tm_F_3 | 59.864 | Size_F_3 | 20 | Tm_R_3 | 59.986 | Size_R_3 | 20 | PRODUCT Size | 263 bp | Primer Start | 10283 | Primer End | 10545 |