Primers Available
Organism:Ophioglossum californicum chloroplast
Repeat:(TAT)4(T)12*
Start:59600 End:59622
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCTGAGTCGTAGGCATGACA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GTTTGGGGAGAGGGATAAGG | |||||||||||||
| Tm_F_1 | 59.855 | Size_F_1 | 20 | Tm_R_1 | 59.761 | Size_R_1 | 20 | PRODUCT Size | 177 bp | Primer Start | 59488 | Primer End | 59664 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCTCCCTGAGTCGTAGGCAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GTTTGGGGAGAGGGATAAGG | |||||||||||||
| Tm_F_2 | 59.827 | Size_F_2 | 20 | Tm_R_2 | 59.761 | Size_R_2 | 20 | PRODUCT Size | 181 bp | Primer Start | 59484 | Primer End | 59664 |
| FORWARD_PRIMER_3 | |||||||||||||
| AATTGCTAGGATCGCACCAT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GTTTGGGGAGAGGGATAAGG | |||||||||||||
| Tm_F_3 | 59.556 | Size_F_3 | 20 | Tm_R_3 | 59.761 | Size_R_3 | 20 | PRODUCT Size | 227 bp | Primer Start | 59438 | Primer End | 59664 |