Primers Available
Organism:Ophioglossum californicum chloroplast
Repeat:(T)10
Start:120677 End:120686
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTCCAGATGAAGCGTAAGCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGATCGAAATCGATGAACCA | |||||||||||||
| Tm_F_1 | 59.978 | Size_F_1 | 20 | Tm_R_1 | 60.009 | Size_R_1 | 20 | PRODUCT Size | 276 bp | Primer Start | 120532 | Primer End | 120807 |
| FORWARD_PRIMER_2 | |||||||||||||
| CAACGGATCCTTTTGTGGAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGATCGAAATCGATGAACCA | |||||||||||||
| Tm_F_2 | 59.79 | Size_F_2 | 20 | Tm_R_2 | 60.009 | Size_R_2 | 20 | PRODUCT Size | 220 bp | Primer Start | 120588 | Primer End | 120807 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTGCTCCAGATGAAGCGTAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGATCGAAATCGATGAACCA | |||||||||||||
| Tm_F_3 | 59.566 | Size_F_3 | 20 | Tm_R_3 | 60.009 | Size_R_3 | 20 | PRODUCT Size | 279 bp | Primer Start | 120529 | Primer End | 120807 |