Primers Available
Organism:Ophioglossum californicum chloroplast
Repeat:(AT)15ac(AT)8
Start:79969 End:80016
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCCCGAAGAATCATGAGATG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CGGGAGCTGTGGAAAGATAG | |||||||||||||
| Tm_F_1 | 60.417 | Size_F_1 | 20 | Tm_R_1 | 59.83 | Size_R_1 | 20 | PRODUCT Size | 277 bp | Primer Start | 79816 | Primer End | 80092 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCCCGAAGAATCATGAGATG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCATTGCAAACTCTCCCTTC | |||||||||||||
| Tm_F_2 | 60.417 | Size_F_2 | 20 | Tm_R_2 | 59.82 | Size_R_2 | 20 | PRODUCT Size | 244 bp | Primer Start | 79816 | Primer End | 80059 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCCCGAAGAATCATGAGATG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GTACGGGAGCTGTGGAAAGA | |||||||||||||
| Tm_F_3 | 60.417 | Size_F_3 | 20 | Tm_R_3 | 60.255 | Size_R_3 | 20 | PRODUCT Size | 280 bp | Primer Start | 79816 | Primer End | 80095 |