Primers Available
Organism:Ophioglossum californicum chloroplast
Repeat:(AGGT)3
Start:105590 End:105601
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AACGACCTTCAACAAGTGGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CGGAGACCTGTGTTTTTGGT | |||||||||||||
| Tm_F_1 | 60.005 | Size_F_1 | 20 | Tm_R_1 | 60.005 | Size_R_1 | 20 | PRODUCT Size | 198 bp | Primer Start | 105547 | Primer End | 105744 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCAAGAAAAGCTCGAACGAC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CGGAGACCTGTGTTTTTGGT | |||||||||||||
| Tm_F_2 | 59.993 | Size_F_2 | 20 | Tm_R_2 | 60.005 | Size_R_2 | 20 | PRODUCT Size | 212 bp | Primer Start | 105533 | Primer End | 105744 |
| FORWARD_PRIMER_3 | |||||||||||||
| AACGACCTTCAACAAGTGGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCCCCATACATGGTCTTACG | |||||||||||||
| Tm_F_3 | 60.005 | Size_F_3 | 20 | Tm_R_3 | 60.066 | Size_R_3 | 20 | PRODUCT Size | 224 bp | Primer Start | 105547 | Primer End | 105770 |