Primers Available
Organism:Ophioglossum californicum chloroplast
Repeat:(AGGG)3
Start:83972 End:83983
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGAACCGCACAAGCATCTTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GAGTATGGAGCGGGTGGTAA | |||||||||||||
| Tm_F_1 | 59.882 | Size_F_1 | 20 | Tm_R_1 | 59.955 | Size_R_1 | 20 | PRODUCT Size | 223 bp | Primer Start | 83843 | Primer End | 84065 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATTAACTTCCCCCAGAACCG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GAGTATGGAGCGGGTGGTAA | |||||||||||||
| Tm_F_2 | 60.18 | Size_F_2 | 20 | Tm_R_2 | 59.955 | Size_R_2 | 20 | PRODUCT Size | 236 bp | Primer Start | 83830 | Primer End | 84065 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATGAAGTCGGTTGATTGGGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GAGTATGGAGCGGGTGGTAA | |||||||||||||
| Tm_F_3 | 60.317 | Size_F_3 | 20 | Tm_R_3 | 59.955 | Size_R_3 | 20 | PRODUCT Size | 266 bp | Primer Start | 83800 | Primer End | 84065 |