Primers Available
Organism:Ophioglossum californicum chloroplast
Repeat:(A)18g(AT)13
Start:27161 End:27205
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GAACCGGAATGACAGGAAGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CGTCCAATGAGGAATAGGGA | |||||||||||||
| Tm_F_1 | 60.05 | Size_F_1 | 20 | Tm_R_1 | 59.887 | Size_R_1 | 20 | PRODUCT Size | 264 bp | Primer Start | 27123 | Primer End | 27386 |
| FORWARD_PRIMER_2 | |||||||||||||
| GAACCGGAATGACAGGAAGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCGTCCAATGAGGAATAGGG | |||||||||||||
| Tm_F_2 | 60.05 | Size_F_2 | 20 | Tm_R_2 | 59.887 | Size_R_2 | 20 | PRODUCT Size | 265 bp | Primer Start | 27123 | Primer End | 27387 |
| FORWARD_PRIMER_3 | |||||||||||||
| GAACCGGAATGACAGGAAGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGTTTTGTCGACCATTACGG | |||||||||||||
| Tm_F_3 | 60.05 | Size_F_3 | 20 | Tm_R_3 | 60.227 | Size_R_3 | 20 | PRODUCT Size | 163 bp | Primer Start | 27123 | Primer End | 27285 |