Primers Available
Organism:Ophioglossum californicum chloroplast
Repeat:(A)12tagagagataaataa(AT)8
Start:1252 End:1294
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGTTTTGAGTGGCAGGGATG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGTTGCTAAAGCTGCCATTG | |||||||||||||
| Tm_F_1 | 60.111 | Size_F_1 | 20 | Tm_R_1 | 60.014 | Size_R_1 | 20 | PRODUCT Size | 270 bp | Primer Start | 1205 | Primer End | 1474 |
| FORWARD_PRIMER_2 | |||||||||||||
| AGTTTTGAGTGGCAGGGATG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTCCGGTAGTATGGGACTGC | |||||||||||||
| Tm_F_2 | 60.111 | Size_F_2 | 20 | Tm_R_2 | 59.955 | Size_R_2 | 20 | PRODUCT Size | 165 bp | Primer Start | 1205 | Primer End | 1369 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATGGAGTTTTGAGTGGCAGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTCCGGTAGTATGGGACTGC | |||||||||||||
| Tm_F_3 | 60.111 | Size_F_3 | 20 | Tm_R_3 | 59.955 | Size_R_3 | 20 | PRODUCT Size | 169 bp | Primer Start | 1201 | Primer End | 1369 |