Primers Available
Organism:Ophioglossum californicum chloroplast
Repeat:(A)12
Start:5991 End:6002
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTGATTTCTGAACCAATCGAGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AACTCAAACATTGGTGGGGA | |||||||||||||
| Tm_F_1 | 59.685 | Size_F_1 | 22 | Tm_R_1 | 60.21 | Size_R_1 | 20 | PRODUCT Size | 210 bp | Primer Start | 5886 | Primer End | 6095 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTGATTTCTGAACCAATCGAGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CTCAAACATTGGTGGGGATT | |||||||||||||
| Tm_F_2 | 59.685 | Size_F_2 | 22 | Tm_R_2 | 59.647 | Size_R_2 | 20 | PRODUCT Size | 208 bp | Primer Start | 5886 | Primer End | 6093 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTGATTTCTGAACCAATCGAGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GTGGATCCCCAAAATTGAAA | |||||||||||||
| Tm_F_3 | 59.685 | Size_F_3 | 22 | Tm_R_3 | 59.599 | Size_R_3 | 20 | PRODUCT Size | 154 bp | Primer Start | 5886 | Primer End | 6039 |