Primers Available
Organism:Ophioglossum californicum chloroplast
Repeat:(A)11
Start:79138 End:79148
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GGCCTTATTCACAGGTTCCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCACCGCGTCGATATTGTAA | |||||||||||||
| Tm_F_1 | 59.933 | Size_F_1 | 20 | Tm_R_1 | 60.096 | Size_R_1 | 20 | PRODUCT Size | 250 bp | Primer Start | 79017 | Primer End | 79266 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGCCTTATTCACAGGTTCCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AAGATCCAATTGATCACCGC | |||||||||||||
| Tm_F_2 | 59.933 | Size_F_2 | 20 | Tm_R_2 | 59.9 | Size_R_2 | 20 | PRODUCT Size | 263 bp | Primer Start | 79017 | Primer End | 79279 |
| FORWARD_PRIMER_3 | |||||||||||||
| GCATCGATTTCAAGGGACAT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCACCGCGTCGATATTGTAA | |||||||||||||
| Tm_F_3 | 59.9 | Size_F_3 | 20 | Tm_R_3 | 60.096 | Size_R_3 | 20 | PRODUCT Size | 187 bp | Primer Start | 79080 | Primer End | 79266 |