Primers Available
Organism:Equisetum hyemale chloroplast
Repeat:(T)21
Start:17863 End:17883
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AACATGAGCTGGACCGGTAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGGTTGCTTTGACTACATCCA | |||||||||||||
| Tm_F_1 | 59.434 | Size_F_1 | 20 | Tm_R_1 | 59.985 | Size_R_1 | 21 | PRODUCT Size | 140 bp | Primer Start | 17769 | Primer End | 17908 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGAGTAAAATCCCGTTCCCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGGTTGCTTTGACTACATCCA | |||||||||||||
| Tm_F_2 | 59.284 | Size_F_2 | 20 | Tm_R_2 | 59.985 | Size_R_2 | 21 | PRODUCT Size | 259 bp | Primer Start | 17650 | Primer End | 17908 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGGGGAGCATGCATTATAGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGGTTGCTTTGACTACATCCA | |||||||||||||
| Tm_F_3 | 59.166 | Size_F_3 | 20 | Tm_R_3 | 59.985 | Size_R_3 | 21 | PRODUCT Size | 199 bp | Primer Start | 17710 | Primer End | 17908 |