Primers Available
Organism:Equisetum hyemale chloroplast
Repeat:(T)10ctcacaacttattttttagttc(T)13gtctaagaactcgactagaacaactatgataaagaagtttctaaggcaagcatgtgatttcg(TTA)4
Start:55113 End:55231
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CGGTGAACGCATAGTCATTG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTTTTAGTTAGGTCCGTTAAGCA | |||||||||||||
| Tm_F_1 | 60.134 | Size_F_1 | 20 | Tm_R_1 | 57.353 | Size_R_1 | 23 | PRODUCT Size | 246 bp | Primer Start | 55022 | Primer End | 55267 |
| FORWARD_PRIMER_2 | |||||||||||||
| GTTCCGGTGAACGCATAGTC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTTTTAGTTAGGTCCGTTAAGCA | |||||||||||||
| Tm_F_2 | 60.526 | Size_F_2 | 20 | Tm_R_2 | 57.353 | Size_R_2 | 23 | PRODUCT Size | 250 bp | Primer Start | 55018 | Primer End | 55267 |
| FORWARD_PRIMER_3 | |||||||||||||
| CGGTGAACGCATAGTCATTG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTTTTAGTTAGGTCCGTTAAGCACT | |||||||||||||
| Tm_F_3 | 60.134 | Size_F_3 | 20 | Tm_R_3 | 58.956 | Size_R_3 | 25 | PRODUCT Size | 246 bp | Primer Start | 55022 | Primer End | 55267 |