Primers Available
Organism:Equisetum hyemale chloroplast
Repeat:(T)10ataattataatagtagatatggtaaaactaggaactttataccattctctaatagtctaaactggatataattaaaacgaatttgaagactaatcaat(A)10
Start:68839 End:68956
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGCAAAGTCCAATAAAAATCGT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGCTGGAAAAACCAAACCAA | |||||||||||||
| Tm_F_1 | 57.059 | Size_F_1 | 22 | Tm_R_1 | 59.587 | Size_R_1 | 20 | PRODUCT Size | 267 bp | Primer Start | 68800 | Primer End | 69066 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTTTAGCAAAGTCCAATAAAAATCG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AGCTGGAAAAACCAAACCAA | |||||||||||||
| Tm_F_2 | 59.928 | Size_F_2 | 25 | Tm_R_2 | 59.587 | Size_R_2 | 20 | PRODUCT Size | 271 bp | Primer Start | 68796 | Primer End | 69066 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGCAAAGTCCAATAAAAATCGT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCTGGAAAAACCAAACCAAC | |||||||||||||
| Tm_F_3 | 57.059 | Size_F_3 | 22 | Tm_R_3 | 59.452 | Size_R_3 | 20 | PRODUCT Size | 266 bp | Primer Start | 68800 | Primer End | 69065 |