Primers Available
Organism:Equisetum hyemale chloroplast
Repeat:(T)10actatttttgtatttctcgataaaaggtcctaatatcttagtaactcacagctaatagtaaaaaaaaagaatttgactaaaatgaatttta(T)12
Start:67232 End:67344
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GGTTCAAATCCCCTCCAACT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTCAAAGATTCAAAAAGTTGGTCT | |||||||||||||
| Tm_F_1 | 60.169 | Size_F_1 | 20 | Tm_R_1 | 58.422 | Size_R_1 | 24 | PRODUCT Size | 277 bp | Primer Start | 67109 | Primer End | 67385 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTCAAATCCCCTCCAACTCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTCAAAGATTCAAAAAGTTGGTC | |||||||||||||
| Tm_F_2 | 60.43 | Size_F_2 | 20 | Tm_R_2 | 57.481 | Size_R_2 | 23 | PRODUCT Size | 275 bp | Primer Start | 67111 | Primer End | 67385 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGTTCAAATCCCCTCCAACT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GTTCAAAGATTCAAAAAGTTGGTCT | |||||||||||||
| Tm_F_3 | 60.169 | Size_F_3 | 20 | Tm_R_3 | 59.172 | Size_R_3 | 25 | PRODUCT Size | 278 bp | Primer Start | 67109 | Primer End | 67386 |