Primers Available
Organism:Equisetum hyemale chloroplast
Repeat:(AGAT)3
Start:128443 End:128454
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AAAAGGCGTTGAGAGACCCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCCTTCGTTTGCTTTTCTTG | |||||||||||||
| Tm_F_1 | 60.247 | Size_F_1 | 20 | Tm_R_1 | 59.996 | Size_R_1 | 20 | PRODUCT Size | 244 bp | Primer Start | 128365 | Primer End | 128608 |
| FORWARD_PRIMER_2 | |||||||||||||
| ACTCTCCGTTGTACTGCGCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AACCACCTACGGATTGATGC | |||||||||||||
| Tm_F_2 | 60.081 | Size_F_2 | 20 | Tm_R_2 | 59.82 | Size_R_2 | 20 | PRODUCT Size | 220 bp | Primer Start | 128268 | Primer End | 128487 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGTGCTTGTTTTCCTTTCCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AACCACCTACGGATTGATGC | |||||||||||||
| Tm_F_3 | 60.088 | Size_F_3 | 20 | Tm_R_3 | 59.82 | Size_R_3 | 20 | PRODUCT Size | 193 bp | Primer Start | 128295 | Primer End | 128487 |