Primers Available
Organism:Equisetum hyemale chloroplast
Repeat:(A)12
Start:20887 End:20898
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTTTTGGGGGATCATCTGAG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AATCGAACCCGCAACATTAG | |||||||||||||
| Tm_F_1 | 59.864 | Size_F_1 | 20 | Tm_R_1 | 59.96 | Size_R_1 | 20 | PRODUCT Size | 224 bp | Primer Start | 20801 | Primer End | 21024 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTTTTGGGGGATCATCTGAG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCAACATTAGCTTGGAAGGC | |||||||||||||
| Tm_F_2 | 59.864 | Size_F_2 | 20 | Tm_R_2 | 59.851 | Size_R_2 | 20 | PRODUCT Size | 214 bp | Primer Start | 20801 | Primer End | 21014 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTTTTGGGGGATCATCTGAG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTGTCTATAGCGGGTAGCGG | |||||||||||||
| Tm_F_3 | 59.864 | Size_F_3 | 20 | Tm_R_3 | 60.244 | Size_R_3 | 20 | PRODUCT Size | 245 bp | Primer Start | 20801 | Primer End | 21045 |